Fig. 5From: Genome-wide QTL and eQTL mapping reveal genes associated with growth rate trait of the Pacific white shrimp (Litopenaeus vannamei)Silencing of candidate trait genes in L. vannamei by RNA interference. (a) Agarose gel electrophoretogram showing in vitro dsRNA synthesis. M: DNA Marker 2000; lane 1: dsRNA-2329_0|path2; lane 2: dsRNA-5952_686|path0; lane 3: dsRNA-C10972/f3p0/1885; lane 4: dsRNA-545_0|path20; lane 5: dsRNA-egfp. mRNA levels of different dsRNAs injected into shrimp, including (b) dsRNA-2329_0|path2, (c) dsRNA-5952_686|path0, (d) dsRNA-C10972/f3p0/1885, and (e) dsRNA-545_0|path20. mRNA levels are shown in comparison to the control group injected with dsRNA-egfp at 0, 24, 48, and 72 h after injection. (f) Comparison of weight gain in L. vannamei 30 days after injection with different dsRNAs. The data were statistically analyzed using paired Student’s t-tests. Asterisks denote statistical significance (*: p < 0.5; **: p < 0.01). (g) Comparison of average body weight between LV-B and LV-S shrimp strains. The data were statistically analyzed using paired Student’s t-tests. Asterisks denote statistical significance (**: p < 0.01). (h) Comparison of the average expression level of Steap4 genes in LV-B and LV-S shrimp strains. The data were statistically analyzed using paired Student’s t-tests. Asterisks denote statistical significance (**: p < 0.01). (i) Correlation between individual body weight and the expression level of Steap4 genes in LV-B strain. (j) Correlation between individual body weight and the expression level of Steap4 genes in LV-S strainBack to article page